Genetic Method To Analyze Essential Genes of Escherichia coli
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چکیده
منابع مشابه
Identification of Biofilm Encoding Genes (agg) in the Escherichia coli Isolates by Multiplex-PCR Method
Introduction: Biofilm is a collection of microbial cells that are irreversibly suppressed and mildly washed away. The aggR gene is located on the main plasmid (pAA), which codes for many actuator factors. The pathogen strains of EAEC have an aggR gene, the gene has been identified in six classes of pathogenic E. coli. Materials & Methods: In the present study, 60 stool samples from children ...
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sucAB and sucCD of Escherichia coli encode enzymes that generate succinyl-CoA from 2-oxoglutarate and succinate, respectively. Their mutual essentiality was studied. sucAB and sucCD could be deleted individually, but not simultaneously. The mutual essentiality of sucAB and sucCD was further confirmed by the conditional expression of sucABCD, sucAB, and sucCD under the control of a P(BAD) in E. ...
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The essential genes of microorganisms encode biological functions important for survival and thus tend to be of high scientific interest. Drugs that interfere with essential functions are likely to be interesting candidates for antimicrobials. However, these genes are hard to study genetically because knockout mutations in them are by definition inviable. We recently described a conditional mut...
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We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene in vitro. For convenience, the deletion can be marked by an antibiotic resistan...
متن کامل-regulated genes in Escherichia coli
Accepted 9 March, 2006. *For correspondence. E-mail pjkiley @wisc.edu; Tel. ( + 1) 608 262 6632; Fax ( + 1) 608 262 5253. Present address: † The Burnham Institute, La Jolla, CA 92037, USA; ‡ Department of Biomolecular Chemistry, University of Wisconsin, Madison, WI 53706, USA. IscR-dependent gene expression links iron-sulphur cluster assembly to the control of O 2 -regulated genes in Escherichi...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2007
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01756-07